High Genomic Diversity of Multi-Drug Resistant Wastewater

Mahfouz, Norhan, Caucci, Serena, Achatz, Eric, Semmler, Torsten, Guenther, Sebastian, Berendonk, Thomas U. and Schroeder, Michael, (2018). High Genomic Diversity of Multi-Drug Resistant Wastewater. Scientific Reports, 8 1-12

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  • Sub-type Journal article
    Author Mahfouz, Norhan
    Caucci, Serena
    Achatz, Eric
    Semmler, Torsten
    Guenther, Sebastian
    Berendonk, Thomas U.
    Schroeder, Michael
    Title High Genomic Diversity of Multi-Drug Resistant Wastewater
    Appearing in Scientific Reports
    Volume 8
    Publication Date 2018-06-12
    Place of Publication Online
    Publisher Springer Nature
    Start page 1
    End page 12
    Language eng
    Abstract Wastewater treatment plants play an important role in the emergence of antibiotic resistance. They provide a hot spot for exchange of resistance within and between species. Here, we analyse and quantify the genomic diversity of the indicator Escherichia coli in a German wastewater treatment plant and we relate it to isolates’ antibiotic resistance. Our results show a surprisingly large pan-genome, which mirrors how rich an environment a treatment plant is. We link the genomic analysis to a phenotypic resistance screen and pinpoint genomic hot spots, which correlate with a resistance phenotype. Besides well-known resistance genes, this forward genomics approach generates many novel genes, which correlated with resistance and which are partly completely unknown. A surprising overall finding of our analyses is that we do not see any difference in resistance and pan genome size between isolates taken from the inflow of the treatment plant and from the outflow. This means that while treatment plants reduce the amount of bacteria released into the environment, they do not reduce the potential for antibiotic resistance of these bacteria.
    Keyword Antibiotic Resistance
    Wastewater Treatment
    Pan-Core genome
    Copyright Holder The Authors
    Copyright Year 2018
    Copyright type Creative commons
    DOI 10.1038/s41598-018-27292-6
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    Created: Fri, 08 Jun 2018, 23:05:04 JST by Claudia Matthias on behalf of UNU FLORES